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KMID : 1094720110160050966
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Biotechnology and Bioprocess Engineering
2011 Volume.16 No. 5 p.966 ~ p.966
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Protective effect of GABA-enriched fermented sea tangle against ethanol-induced cytotoxicity in HepG2 Cells
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Kang Young-Mi
Qian Zhong-Ji
Lee Bae-Jin
Kim Young-Mog
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Abstract
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This study was conducted to evaluate the protective effects of GABA (gamma-amino butyric acid)-enriched sea tangle juice (STJ) by Lactobacillus brevis BJ-20 fermentation against alcohol hepatotoxicity. The protective effects were determined by assessing glutathione (GSH) content levels and gamma-glutamyl transpeptidase (GGT) activity against ethanol-induced cytotoxicity in HepG2 cells. In ethanol-treated cells, GSH content decreased to 44.35% of control (ethanol-untreated cell) values; however, treatment with fermented sea tangle juice (FSTJ) at a concentration of 25 μg/mL increased GSH levels to 67.08%.These results suggest that FSTJ may prevent intracellular GSH depletion caused by ethanol consumption. Treatment with FSTJ against alcohol-injured HepG2 cells resulted in a dose-dependent decrease in GGT activity. The expression of cytochrome P450 2E1 (CYP2E1) enzyme, a major contributor to ethanol-induced oxidative stress, was also completely inhibited in FSTJ-treated cells at a concentration of 25 μg/mL. Thus, this study demonstrated that ethanolinduced cytotoxicity could be attenuated by inhibition of GSH depletion, GGT activity, and CYP2E1 expression.
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KEYWORD
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alcohol hepatocytotoxicity, gamma-glutamyl transpeptidase, fermented sea tangle juice, HepG2 cells
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